Cell Autofluorescence and Lipofuscin

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Cell Autofluorescence and Lipofuscin

I was greatly impressed by an article recently published in Nature Methods (1), in which intracellular autofluorescence was characterized as a biomarker for epithelial cancer stem cells (CSCs). Interestingly, this epithelial CSC-specific autofluorescence was also associated with a highly invasive and chemoresistant phenotype. Riboflavin (vitamin B2) – an ABCG2-selectively transported substrate ...

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Lipofuscin, Lipofuscin-Like Pigments and Autofluorescence

A brief overview is here provided on lipofuscin and lipofuscin-like substances, with particular reference to their biological significance as well as to their cellular origin and pathophysiological role. Special emphasis is also placed on the mutual relationships between lipofuscin and lipofuscin-like lipopigments on one side, and cell autofluorescence on the other.

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Fundus Autofluorescence and RPE Lipofuscin in Age-Related Macular Degeneration

Genes that increase susceptibility to age-related macular degeneration (AMD) have been identified; however, since many individuals carrying these risk alleles do not develop disease, other contributors are involved. One additional factor, long implicated in the pathogenesis of AMD, is the lipofuscin of retinal pigment epithelium (RPE). The fluorophores that constitute RPE lipofuscin also serve ...

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Lipofuscin-associated photo-oxidative stress during fundus autofluorescence imaging

PURPOSE Current standards and guidelines aimed at preventing retinal phototoxicity during intentional exposures do not specifically evaluate the contribution of endogenous photosensitizers. However, certain retinal diseases are characterized by abnormal accumulations of potential photosensitizers such as lipofuscin bisretinoids in the retinal pigment epithelium (RPE). We sought to determine the...

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The accumulation of lipofuscin in the retinal pigment epithelium (RPE) is dependent on the effectiveness of photoreceptor outer segment material degradation. This study explored the role of autophagy in the fate of RPE lipofuscin degradation. After seven days of feeding with either native or modified rod outer segments, ARPE-19 cells were treated with enhancers or inhibitors of autophagy and th...

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ژورنال

عنوان ژورنال: Frontiers in Medicine

سال: 2015

ISSN: 2296-858X

DOI: 10.3389/fmed.2015.00006